Developmental Biology

Developmental Biology

Imaging the internal structure of control or model organisms can lead to better understanding in arthritis, drug development, and physiology. Research in developmental biology often begins by observing phenotype differences in control and mutants in the embryos and larvae of model organisms such as mice and zebrafish. For example, understanding developmental processes requires accurate visualization and parameterization of three-dimensional embryos. ZEISS X-ray microscopy (XRM) provides quantitative non-destructive 3D tomography for unique opportunities for 3D phenotyping. The ability to work with intact specimens and being able to probe selected regions help address the current limitations in histology and serial sectioning, which are destructive techniques that are time consuming and susceptible to artifacts. XRM is a powerful advanced characterization technique that can help bridge the current sample size, sample thickness, sample opaqueness gap and complement established imaging modalities such as confocal microscopy, histology and electron microscopy.

Characterization and Analysis

  • Understand modular formation of tendon-bone attachment unit by a distinct pool of progenitors
  • Deliver 3D volumetric and distribution measurements of anatomical and cellular features including eye, nerve, brain, and heart cells in zebrafish
  • Perform in situ compressive loading and correlative noninvasive imaging of the bone-periodontal ligament-tooth fibrous joint
  • Incorporate propagation phase contrast to perform quantitative imaging of murine osteoarthritic cartilage
  • Enable murine lung morphometry for detailed structural evaluation
  • Assist comparative developmental studies, embryo phenotyping, and quantitative modeling of development

Systems

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ZEISS Xradia 520 Versa

High resolution, non-destructive 3D X-ray microscope with spatial resolution <700 nm (70 nm minimum voxel size), and resolution at a distance, with absorption and phase contrast enabled by a unique dual magnification system
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ZEISS Xradia 810 Ultra

ZEISS Xradia 810 Ultra
Nanoscale X-ray Imaging:
Spatial resolution <50 nm (16 nm minimum voxel size) with Zernike phase contrast for high resolution, non-destructive imaging of biological samples in the lab.
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Publications
Keywords
murine, mammalian, embryology, teratology, genetic research, morphometry, phenotyping